Higher specific activities of alkaline phosphatase and β-galactosidase for the cellular membrane integrity in young and old aged lithium treated rats

• Author Details:   
• Prabha M
• Sushma KN
• Suneetha P
• RR Siva Kiran

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Background: Differentiate the specific activity of Alkaline phosphatase and β-galactosidase in LiCl₂ treated young and old rat's brain and liver to their control.
Material and Methods: Totally 4 groups male wistar rats (each n=5) are taken to assess the level of Alkaline phosphatase and β-galactosidase for 10 and 20 days LiCl₂ treated young and old rat's brain and liver as compared to control.
Results: LiCl₂ increases protein content essentail for old brain cell activation. Among all four group rats Alkaline phosphatase and β-galactosidase showed increased specific activity for 10 and 20 days LiCl₂ treated young and old rat's brain and liver as compared to their control. Infact Alkaline phosphatase showed significantly higher activity for 10 and 20 days LiCl₂ treated young brain (16.77 ± 0.309 and 27.74 ± 0.237) than their control 3.80 ± 0.230 and old rat's brain (14.28 ± 0.215 and 16.30 ± 0.24) than their control 8.65 ± 0.304 nm/min/mg of protein, similarly higher activity obtained for young liver (19.51 ±0.487 and 21.28 ± 0.317) than their control 3.67 ± 0.402 and finally for the old liver (15.02 ± 0.355 and 18.45 ± 0.439) as compared to their control 3.26 ± 0.304 nm/min/mg of protein respectively.
Conclusion: These results confirm both membrane bound enzymes showed higher specific activity for LiCl₂ treatment in brain and liver cells. So lithium has positive effect on young and old brain cells for β-galactosidase and has more positive effect on Alkaline phosphatase for rat liver and brain for membrane integrity of cells.

Keywords: Total protein, Alkaline phosphatase, β-galactosidase, Specific activity, Lithium Chloride